Partnership for the Advancement of Cancer Research Project
This pilot project will investigate functional interactions between two signaling proteins common to the PI3-Kinase signaling pathway from organisms as diverse as C. elegans and man. The Grb7 (MIG10-family) protein has been studied in the Lyons laboratory while the s-SHIP product has been a focus of research in the Rohrschneider laboratory. Grb7 is a downstream messenger protein important in PI3-kinase signaling, while s-SHIP is important in down-regulation of PI3-kinase signaling pathways. Each protein may have a role in mammary tumor formation or maintenance, yet little, or nothing, is known about their interactions in mammary tumors or their stem cells. Recently, work in the Rohrschneider lab has identified and isolated normal mammary tissue stem cells from a transgenic mouse model. The work on this pilot project will combine expertise from both labs to investigate how each protein may function in mammary tissue stem cells. The proposed molecular analyses of signaling protein interactions in mammary stem cells has not previously been possible. This project represents a novel combined approach derived from the distinct interests of the Lyons and Rohrschneider laboratories.
The specific aims of this pilot project are to:
Information about Dr. Lyons' research can be found at: www.chemistry.nmsu.edu/lyons.html