• Spin down 1.5 ml of cells from O/N culture
• Resuspend in 1 ml 70% or 100% EtOH and rotate O/N
• Spin out of EtOH
• Resuspend in 1 ml H2O
• Spin and resuspend in 0.5 ml 50 mM Tris pH 8.0
• Add 10 µ l RNase A (10 mg/ml), incubate 2 - 4 hrs at 37°C
• Spin and resuspend in 0.5 ml 50 mM Tris pH 7.5 containing 2 mg/ml proteinase K
• Incubate 30 - 60 min at 50°C
• Spin out of protease solution
• Resuspend in 0.5 ml FACS buffer
• Transfer 100 µ l (or more if low # of cells) to another tube, add 1 ml of 1X Sytox (stock is 5000x) in 50 mM Tris pH 7.5
• Sonicate at setting 3 for 4 sec then analyze

 FACS-buffer:
200 mM Tris/HCl pH 7.5; 200 mM NaCl; 78 mM MgCl

5000x Sytox Solution:
5 mM Sytox Green in DMSO (Molecular Probes S-7020)