Mutant Strains
Figure: p27 -/- mice (right) are heavier than their wildtype littermates (left)
p27KO
p27 Knockout mice generated at the Fred Hutchinson Cancer Research
Center
are currently being provided to the research community by the Induced
Mutant
Resource of the Jackson Laboratories. Please see their web
page for details on availability. Unlike Jax, which carries C57BL/6J,
the Fero lab also maintains p27KO mice on the 129S4
background. If you are in need of information
or materials that Jax can not provide please feel free to contact
us.
POMC-Cre
The proopiomelanocortin (POMC) gene promoter drives expression of the Cre recombinase in this transgenic
mouse model. This leads to Cre
expression in cells of the pars
intermedia, and to a lesser extent pars distalis of the pituitary, and the
hypothalamus. Maintained on the 129S4 and C57BL/6J
background. This model is has been shown to be efficient and
highly selective to POMC Cre expressing cells, particlulary the pars intermedia melanotrophs.
p27Kfl
The p27 locus has been targeted with LoxP sites in order to
create inducible or tissue specific p27 gene deletion. This model
has been validated with POMC Cre and tested with other systems.
p27Ksnl
LoxP sites and a STOP cassette were used to create a targeted
null allele of p27 which can be converted to a functional allele driven
by the endogenous prometer in an inducible or tissue specific
pattern. This model has been validated with POMC Cre and tested
with other systems.
p27Kl
The product of germline Cre expression in p27Kfl mice is a targetted
deletion of p27 without the presence of a neomycin selectible
marker. Useful in cells or tissues where constitutive Neo expression is
problematic. This model is undergoing validation.
References:
- Chien WM, et al. Genetic mosaics reveal both cell-autonomous and cell-nonautonomous function of murine p27Kip1. Proc Natl Acad Sci USA. 2006 103(11):4122-7.
- Fero ML, et al. A syndrome of multiorgan hyperplasia with features of gigantism, tumorigenesis, and female sterility in p27(Kip1)-deficient mice. Cell. 1996 May 31; 85(5): 733-744.
- Fero ML, et al. The murine gene p27Kip1 is haplo-insufficient for tumour suppression. Nature. 1998 Nov 12;396(6707):177-80.
- Lowenheim H, et al. Gene disruption of p27(Kip1) allows cell proliferation in the postnatal and adult organ of corti. Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4084-8.
Protocols:
Tail DNA prep
Genotyping p27 knockout mice
