Solutions for Hartwell's Complete (HC) Media

1. 10X HC (7 amino acid dropout solution)
2. 10X YNB solution
3. Amino acid solutions
4. HC plate recipe
5. FOA plate recipe

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10X HC (7 Dropout Amino Acid Liquid)

2.4 g tyr
3.2 g ile
2.0 g phe
4.0 g glu
8.0 g thr
4.0 g asp
6.0 g val
16.0 g ser
0.8 g arg

in final volume of 4L - Autoclave

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10X YNB

58 g YNB (w/o amino acids and w/o ammonium sulfate)
200 g ammonium sulfate

in a final volume of 4L - Autoclave

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Amino Acid Solutions (note these are NOT 10X solutions, see below)

URA 1 mg/ml
ADE 1 mg/ml
LYS 10 mg/ml
MET 2 mg/ml
TRP 10 mg/ml
LEU 20 mg/ml
HIS 10 mg/ml

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Example Recipe of Complete Plates

20 g of Agar in 609 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:

100 ml	20% sterile glucose
100 ml	10X YNB solution
100 ml	10X HC-7aa dropout solution
35 ml	URA solution
20 ml	ADE solution
12 ml	LYS solution
10 ml	MET solution
8 ml	TRP solution
4 ml	LEU solution
2 ml	HIS solution

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FOA plates

20 g of Agar in 609 ml distilled water in a 2L flask
Autoclave 20 minutes

Add the following:

100 ml	20% sterile glucose
100 ml	10X YNB solution
100 ml	10X HC-7aa dropout solution
35 ml	URA solution
20 ml	ADE solution
12 ml	LYS solution
10 ml	MET solution
8 ml	TRP solution
4 ml	LEU solution
2 ml	HIS solution

Let flask cool to 50 deg C, then add 1.0 g of 5-FOA.

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