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Simon Lab at the FHCRC

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Yeast Screen of Anticancer Agents

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These data have not been endorsed by the National Cancer Institute or the Fred Hutchinson Cancer Research Center.

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Drug profiles on the yeast strain panel were obtained as follows:

• Cells were continuously exposed to drugs during 7 generations of mid-log to early plateau phase growth.
• Cells were exposed to 7 concentrations of serially two-fold diluted drugs (in the appropriate concentration range).
• Incubations were carried out in triplicate in 96-well plates.
• Cell growth was measured by OD₆₆₀ or colony forming unit (cfu) determination as indicated.
• IC₅₀ values on each strain were derived from 3 independent experiments and are shown as the log of the molarity. The vertical bar is set at the wild type value. An asterisk indicates that the drug was not soluble at a high enough concentration to obtain an IC₅₀ value on the particular strain.

The profile of sensitivity of the yeast strain panel to X-rays was obtained as follows:

• Yeast cultures were grown in liquid YC.
• Varying numbers of cells were plated onto YEPD plates.
• Cells were irradiated from 10 to 400 seconds at 106 rads per second.
• Cells were incubated at 30oC for 2 days. Subsequently, colony forming units (cfu) were determined and the percentage of surviving cell was calculated using un-irradiated control plates.

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