Glucuronidation in Humans:
Genotype and Phenotype (DIGEST)

PI: Johanna Lampe PhD

DIGEST website: http://www.fhcrc.org/phs/digest

Research has shown that certain fruits and vegetables can increase the activity of a group of enzymes (called glucuronosyltransferases or UGT for short) that help our body get rid of potentially harmful substances, including carcinogens. Genetic differences among individuals can play a role in how effectively these cancer-fighting enzymes work. The DIGEST study will look at how the interplay of genes and diet – in particular, a diet rich in certain fruits and vegetables – may affect the function of the body's cancer-fighting enzymes. The results of this study, funded by the National Cancer Institute, could be important in making recommendations about diets that prevent cancer.

About 300 people, ages 20 to 40, are sought for the first half of this two-part study. They will be asked detailed questions about diet and health. DNA from a sample of blood will be analyzed to determine genetic patterns of enzymes. Activity of these cancer-fighting enzymes will be measured by looking at the rate that the body breaks down aspirin and Tylenol.

Of those initially enrolled, 60 will be asked to participate in the second part, two 14-day feeding studies during which the Hutchinson Center will provide all food and beverages. Eligibility will depend on genetic patterns of the enzymes.

For more information, contact Yvonne Schwarz, or email DIGEST@fhcrc.org