Equipment

Microscopes

• The JEOL 1010 transmission electron microscope is equipped with a 1K x 1K Bioscan Gatan wide angle slow scan CCD camera. The microscope has a 100kV accelerating voltage, and a magnification range of 50X - 500,000X.
  
  See steps for using the JEOL 1010 microscope and Gatan CCD digital camera.
  
  
  
  
  
  
• The JEOL 1230 transmission electron microscope is equipped with the 2K x 2K ultrascan 100 bottom mount Gatan CCD camera for high resolution work and an 11 Megapixel SC1000 ORIUS side mount Gatan CCD Camera for large field of view work. The microscope has a 120kV accelerating voltage with a magnification range of 50X - 600,000X.
  
  See steps for using the JEOL 1230 microscope and Gatan CCD.
  Loading the sample holder into the JEOL 1230 microscope
  
  
  
• The JEOL 5800 scanning electron microscope is equipped with the JEOL Orion digital image acquisition system, multi-stub holder, and magnification range of 18X - 300,000X.
  
  Steps for using the JEOL 5800 microscope and Orion digital imaging system.
  
  

Cryo Equipment

The Leica cryo equipment includes a High Pressure Freezing apparatus, freeze substitution system, and low temperature cryochamber. This equipment produces superior cellular preservation for morphological studies and better antigen retention for immunolabeling work.

• The Leica EM PACT2 is a high pressure freezing machine that is designed to freeze samples up to 200-300 um into the specimen without significant ice crystal damage. HPF provides for better antigen retention for immunolabeling. HPF is followed by freeze substitution or cryo-ultramicrotomy.
• The Leica EM AFS2 is capable of freeze substitution, progressive lowering of temperature techniques, and low temperature embedding and polymerization of resins for improved preservation of ultra structure and antigenicity.
• Cryo-ultramicrotomy is performed by the Leica EM FC6 cryochamber. It is designed for low temperature sectioning of samples at temperatures from -15 to -185 degrees C.

More information on the equipment is found at the Leica web site.

Support Equipment

• Beckman Airfuge
• Ted Pella Research Microwave System
• Pelco Low-Temperature UV Polymerization System
• Denton Desktop Vacuum Evaporator
• Tousimis Critical Point Dryer
• Denton Desk II Sputter Coater
• Leica EMUC6 and UCT Ultra-Microtomes
• Olympus BX41 Double Headed Light Microscope with Canon digital camera
• Zeiss Dissecting Microscope
• Durst Enlarger
• Ilford Paper Processor
• Epson Expression 1680 Scanner

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