FHCRC Chicken 13K Array

The chicken 13K array was described in the publication:
Development of a cDNA array for chicken gene expression analysis
BMC Genomics. 2005 Feb4; 6(1):13

BACKGROUND: The application of microarray technology to functional genomic analysis in the chicken has been limited by the lack of arrays containing large numbers of genes. RESULTS: We have produced cDNA arrays using chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. From a total of 363,838 chicken ESTs representing 24 different adult or embryonic tissues, a set of 11,447 non-redundant ESTs were selected and added to an existing collection of clones (4,162) from immune tissues and a chicken bursal cell line (DT40). Quality control analysis indicates there are 13,007 useable features on the array, including 160 control spots. The array provides broad coverage of mRNAs expressed in many tissues; in addition, clones with expression unique to various tissues can be detected. CONCLUSIONS: A chicken multi-tissue cDNA microarray with 13,007 features is now available to academic researchers from chicken13k@fhcrc.org. Sequence information for all features on the array is in GenBank, and clones can be readily obtained. Targeted users include researchers in comparative and developmental biology, immunology, vaccine and agricultural technology. These arrays will be an important resource for the entire research community using the chicken as a model.

• Ordering Microarrays
• Microarray Request Form
• Terms and Conditions of Use
• Annotation
• Readme for Chicken 13K Microarray Annotation v3.0
• Annotation file for Chicken 13K Microarray v3.0
• Release Notes
• Protocols
• Ambion Message Amplification
• Coverslip Cleaning
• Hybridization and Washing
• Modified Amplification of RNA_aa-MessageAmp
• Microarray Request Form

If you have questions about using the chicken 13K array, please contact the genomics resource and use Chicken 13K Array in the subject line.