HPLC

Purification, fractionation, and qualitative/quantitative assays of proteins and peptides are performed in the facility by reverse-phase and cation-exchange HPLC. Inquiries into the use other separation modes should be made with the Proteomics Facility.

Sample Preparation

Sample preparation involves SDS-PAGE gel electrophoresis, digestion, and desalting of gel slices. Investigators can take their samples through the complete preparation, or the proteomics staff can assist with some steps. If you choose to prepare your own gel samples, see our Tips for SDS-PAGE Gel Handling

Sample Submission

If you are using a FHCRC project id for billing, please fill out the Internal Submission Form and submit with your sample.

If billing is to be submitted to the University of Washington or other external institution, fill out the External Submission Form and submit with your sample.

Results

Data generated within the resource are transferred to servers maintained by Shared Resources Research Computing Support in the researcher's proteomics folder in their 'fred' account.