Translational Research Program

Affinity Based Strategies to Fast Track Development of Colon Cancer Biomarkers

Principal Investigator: Paul Lampe, PhD

Background:  This project utilizes high-density antibody microarrays to perform both broad proteomic and glycomic screens and specifically targeted analyses to discovercolon cancer biomarkers. We are analyzing both biomarkers of early detection and diagnosis in a 3-stage process: discovery, pre-validation and validation. Our high throughput, 2-stage discovery methodology will rapidly assess the utility of these markers alone, together and in combination with existing markers. 

Specific Aims:

1. To evaluate the expression/activation of proteins important in proliferation, angiogenesis, and inflammation in plasma collected 0-24 months prior to diagnosis of colon cancer.

2. To evaluate changes in the glycosylation status of proteins during colon cancer progression in plasma collected 0-24 months prior to diagnosis.

3.  To develop CRC-specific diagnostic biomarkers using pretreatment cancer tissue and plasma from colorectal cancer patients. 

4. To validate colon cancer early detection and diagnostic biomarkers on independent sample sets.

Fred Hutchinson Cancer Research Center is a world leader in research to prevent, detect and treat cancer and other life-threatening diseases.